Biyernes, Hulyo 25, 2014

Sampa-Sampalukan

Sampa-Sampalukan 


Common Names:   Stone-breaker (Engl.) 
                                     Sampa- sampalukan (Tagalog)
                                     Chanca Peidra (Spanish)

Scientific Name:      Phyllantus nimuri
Family Name:          Phyllantaceae

Description of plant and parts: 

Annual Herb: 30-60 cm high, quite glabrous, stem often branched at the base.
Leaves: Numerous, sbsessile distichous often imbricating, elliptic oblong obtuse. Stipules present, very acute. 
Flowers: Yellowish, very numerous, axillary. The male flowers are one to three in number while the female flowers are solitary in nature. 
Capsules: 2.5mm in diameter, depressed globose, smooth scarcely lobed. 

 
Phyllantus ninuri plant and leaves


Phyllantus ninuri flowers 


Phyllantus ninuri capsules

ACTIVE CONSTITUENTS
1. Kaempferol-4-o-alpha-L-rhamnoside, Aerial plant 0.9%, Root Culture
2. (-) Limonine, Leaf Essential oils 4.5%
3. Ascorbic acid, Leaf 0.41%
4. Cymene, Leaf Essential Oil 11%
5. Hypophyllanthin, Plant 0.05-0.17%
6. Geranin, Plant .23%
7. Linoleic acid, Seed Oil 21%
8. Linolenic acid, Seed Oil 51.4%
9.Ricinoleic acid, Seed Oil 1.2%
10.Phyltetralin, Plant, Leaf 0.14%
11.&Phyllanthin, Leaf, Aerial plant

The following are parts of the plant:
1. (-)-Nor-secrurinine
2. 4-Hydroxy-sesamin
3. Corilagin
4. Ellagic acid
5. Estradiol
6. Fisetin-41-O-beta-D-dlucoside
7. Hinokinin
8. Iso-lintetralin
9. Nirurin
10.Nirurinetin
11.Phyllanthus
12.Trans-phytol
13.Repandusinic acid A

Following are parts of the Roots:
1. (+)-Catechin
2. (+)-Gallocatechin
3. (-)-Epi-catechin
4. (-)-Epi-catechin-3-gallate
5. (-) Epi-gallocatechin
6. Gallic acid
7. (-)-Epi-gallocatechin-3-O-gallate
8. Eriodictyol-7-o-alpha-L-rhamnosidet
9. Fisetin-41-O-alpha-L-rhamoniside
10.Lupeol acetate
11.Lupeol
12.Nor-securinine

Following are parts of the Leaves:
1. 4-Hydroxy-lintetralin
2. 2,3-dimethoxy-iso-lintertralin
3. Astragalin
4. Beta sitosterol
5. Demethylenedioxy niranthin
6. Hydroxy niranthin
7. Hypophyllanthin
8. Iso-quercitin
9. Linnanthin
10.Lintetralin
11.Niranthin
12.Quercitrin
13.Salicylic acid methyl ester
4.Seco-4-hydroxy-lintetralin

Following are parts of the aerial plant:
1.24- Isopropyl Cholestrol
2.Dotriacontanoic acid
3.Nirphyllin
4.Nirurine
5.Phyllanthenol
6.Phyllantheol
7.Phyllester
8.Phyllinurin
9.Phylltetrin
10.Triacontan-1-al
11.Triancontan-1-ol

Following are present in all leaf, stem, aerial plant, roots
1.Nirtetralin,
2.4-Methoxy-nor-securinine
3.Rutin
4. Phyllanthine
5. Phyllochrysine
6. Quercetin

TRADITIONAL USES

- Decoction of entire plant used as tonic for the stomach.
- Bitter fruit used for tubercular ulcers, wounds, sores, scabies, and ringworm. 
- Used for kidney stones and gallstones.
- Fresh root used as remedy for jaundice. 
- Used as emmenagogue and febrifuge. 
- Also used for genitourinary problems: renal colic, cystitis, prostate problems, jaundice, constipation, dyspepsia, gonorrhea.
- Young leaves used for fevers.
- Chewing of fresh leaves used for hiccups.
- Used for baths in newborns.
- Decoction used for coughs in infants.
- Infusion of root and leaves used as tonic and cold, taken cold in repeated doses.

BIMINI
Hot water extract of the entire plant is administered orally, to reduce fevers, and as a laxative. 17
DOMINICAN REPUBLIC
Hot water extract of leaves is administered orally as a popular fever remedy. 18
FIJI
Decoction of dried leaves and roots is taken orally for fever, and for good health.
Dried entire plant, grounded in buttermilk is administered orally for jaundice. Fresh leaf juice is used externally for cuts and bruises. For eye diseases the juice is mixed with castor oil and applied to the eye. Infusion of dried leaves is administered orally for dysentery and diarrhea. Infusion of green root is taken orally to treat heavy menstrual periods. 19
FRENCH GUYANA
Hot water extract of leaves is administered orally as a cholagogue. 20
HAITI
Decoction of dried leaves is taken orally for or used in bath for fever, and orally for indigestion. Decoction of roots are used for stomach aches.

Hot water extract of dried entire plant is administered orally as a spasmolytic and is also against fever. 
21
INDIA
Fresh plant juice is taken orally for genito urinary disorders .
The fruit is used externally for tubercular ulcers, scabies and ringworm .
Hot water extract of dried entire plant is administered orally for diabetes .
For asthma in bronchitis, leprosy, anemia hiccups and as diuretic ayurvedic medicine. 22


KONKAN
Rubbed down with rice-water and used as a remedy for menorrhagia. 23

PAPAU-NEW GUINEA
Fresh leaf juice or fresh root juice are taken orally for venereal diseases. Decoction of dried entire plant is administered orally to treat venereal diseases.
Decoction of dried leaf when taken orally is a treatment for diarrhea. A cupful of leaf decoction is drunk daily. 24
PHILIPPINES
Decoction of dried entire plant is used as a bath for newborns. It is believed to remove disease-causing elements from the skin. Orally the decoction is used for coughs in infants. 25
PUERTO RICO
Hot water extract of leaf and stem is taken orally for fevers.
Infusion of young shoots and leaves are given for dysentery.
Salted poultice of leaves are used for scabby affections; without salt, applied to bruises and wounds; and made with rice water, poultice lessens edematous swellings and ulcers. 26 


 SIND
Roots, leaves, and young shoots are much employed in gonorrhea and other genito-urinary affections. Bark is used as purgative. Milky juice is applied to offensive sores. 27


TANZANIA
Hot water extract of fresh entire plant is administered orally for gonorrhoea. 28
THAILAND
Hot water extract of commercial sample of the entire plant, is administered orally as an antipyretic. 29
 Hot water extract of dried aerial parts administered orally is used as a diuretic, as an antipyretic, and for malaria.
 Hot water extract of dried entire plant is administered orally as an anti-inflammatory agent. 30
VIRGIN ISLANDS
Hot water extract of the plant is taken orally to increase the appetite. 31
WEST INDIES
Hot water extract of roots together with hot water extract of Citrus aurantifolia roots is taken orally to increase appetite. Hot water extract of entire plant administered orally, is taken for malarial fever. The plant is boiled and the tea taken. Water extract of the leaves and roots is taken orally for diabetes, and as a diuretic .
Phyllanthus niruri is used as a diuretic in dropsical affection, gonorrhoea and   other troubles of genito urinary tract. Herb is bitter, astringent, diuretic and febrifuge, antiseptic. Fresh root is a remedy for jaundice. Infusion of young shoots given in dysentery. Leaves are popular remedy against fever. It can be used to increase the appetite and locally to relieve inflammations. It can also be used in case of anorexia. 32

PHARMACOLOGICAL ACTIVITY WITH CLINICAL BASIS

I.Hepatoprotective Effect –

1.        Hepatitis B is one of the major diseases inflicting human population. Conventional treatment with interferon – alpha is very expensive and has many serious side effects. Alternative herbal medicine using extracts of Phyllanthus niruri and Phyllanthus urinaria have been reported to be effective against Hepatitis B and other viral infections. A study reports quantitative determination of the antiviral effect of these herbs in well-defined in vitro systems. 3
2.    Phyllanthus niruri has been reported to exhibit marked antihepatitis B virus surface antigen activity in in-vivo and in-vitro studies. Infectious hepatitis is due to the inability of the bodies’ immune system to eliminate the virus from the liver cells: hence the “carrier state”. An infection with the virus is documented by detectable levels of various viral antigens in the blood, including HbaAg (the surface antigen of the virus) as well as antibodies to the core of virus (HBc antibodies). In one study, 37 patients with chronic viral hepatitis B were treated with a daily dose of 600mg of Phyllanthus niruri for 30 days. 59% of the patients lost the HBsAg two weeks after the end of the treatment. Furthermore, none of the cases followed for up to 9 months had any symptoms of HBsAg. The authors postulated that Phyllanthus nirurimight inhibit proliferation of the virus by inhibiting replication of the genetic material of the virus. 4
3.    Hepatoprotective effect of an ayurvedic medicine; herbal preparation HPN – 12 (containing Glycirrhiza glabr, Pichorhiza kurroa, Berberis aristata, Piper longum, Phyllanthus niruri, Solanum dulcamara, Zingiber officinale, Curculigo orchioides, Elettaria cardamomum, Tinospora cordifolia, Desmodium trifolium and Sacchrum officinarum) orally administered to male albino rats at 1ml/100g body weight was found to be effective against liver damage. 5
4.     Animals with Carbon Tetrachloride induced hepatopathy were treated with catliv (contains extracts of Swertia chirata, Eclipta alba, Fumaria vaillanti, Picorrhiza kurroa, Andrographis paniculata and Phyllanthus niruri) at 25ml twice daily orally for six days starting at 48 hours after administration of Carbon tetrachloride. On basis of result obtained it was concluded that the ingredients in catliv, effectively helped in regeneration of hepatic cells and is an effective liver tonic for calves.6
5.     Research in Japan and India in the 1980's has demonstrated the liver -healing properties of Phyllanthus niruri.  The primary compounds responsible are phyllanthin, hypophyllanthin and triacontanal. Glycosides found in Phyllanthus niruri demonstrated Aldose reductase (AR) inhibitory activity in studies conducted by a Japanese research group in 1988 and 1989.7

 

II.HIV Replication Inhibition–

1.      Aqueous extract of Phyllanthus niruri is reported to have inhibitory effect on human immunodeficiency virus. The investigation examines the anti-HIV effects of the alkaloidal extract of Phyllanthus niruri in human cell lines. The inhibitory effect on HIV replication was monitored in terms of inhibition of virus induced cytopathogenecity in MT-4 cells. The alkaloidal extract of Phyllanthus niruri showed suppressing activity on strains of HIV-1 cells cultured on MT-4 cell lines. The CC50 for the extract was found to be 279.85μgmL-1 whereas the EC50 was found to be 20.98μgmL-1. Interestingly the Selectivity Index (SI) was found to be 13.34, which showed a clear selective toxicity of the extract for the viral cells. The alkaloidal extract of Phyllanthus niruri was thus found to exhibit sensitive inhibitory response on cytopathic effects induced by both the strains of human immunodeficiency virus on human MT-4 cells in the tested concentrations.8
2.       Extracts of five medicinal plants: Aristolochia indica, Cassia occidentalis, Phyllanthus niruri, Withania somnifera and Tinospora cordifolia were administered to 10 HIV infected patients for a period of six months to one year. The clinical status of the patient and their CD4 cell counts were periodically monitored. The results indicate that in seven of the ten patients, their CD4 count increased and the patients remained either asymptomatic or their clinical well being improved. There was no change in the CD4 cell count in one of the patient and the other two progressed to full blown AIDS.9

 

III. Lipid Lowering Activity  -

1.      Lipid lowering activity of Phyllanthus niruri alcoholic extracts in triton induced hyperlipidaemia was examined in rats. It was observed that administration of triton in rat caused increase in serum cholesterol by 3.5 fold, phospholipid 2 fold and triglyceride 1.2 fold. Administration ofPhyllanthus niruri at the dose of 200mg/kg simultaneously with triton lowered the level of total cholesterol, phospholipid and triglyceride by 27, 25 and 24 percent respectively. In an experiment with cholesterol fed rats, Phyllanthus niruri at a dose of 100 mg/kg lowered the elevated level of low-density lipoprotein lipids in hyperlipidemic and drug fed animals.10

IV.Anti – Diabetic Activity –

1.      An alcoholic extract of Phyllanthus niruri was found to reduce significantly the blood sugar in normal rats and in alloxan diabetes rats. In normal rats, administration of Phyllanthus niruri 200mg/kg body weight reduced the blood sugar by 34.5 percent and to 47.4 percent at the concentration of 1000mg/kg by weight at 1 hour. However at 6thhour, values are almost similar to normal value. Continuous administration of the drug produced significant reduction in normal blood sugar in rats, which on 15th day was also found to reduce the blood sugar in alloxan diabetic rats. In short term experiment, drug was found to reduce the blood sugar at 4th hour by 6.07 percent at dose level of 200mg/kg by weight and 18.7 percent at concentration of 1000mg/kg by weight. Continuous administration of drug produced significant reduction in blood sugar in alloxan diabetic rats. On 15th day values were almost similar to normal in the group taking 1000mg/kg by weight. Plant extract did not produce any toxicity as seen from liver and kidney function test and in hematological parameters. The results indicate potential antidiabetic action of Phyllanthus niruri.11

 

V. Anti Malarial Activity –

1.      The ethanolic, dichloromethane and lyophilized aqueous extracts of Cassia occidentalis root bark, Morinda morindoides leaves and whole plants of Phyllanthus niruri were evaluated for their antimalarial activity in vivo, in 4-day, suppressive assays against Plasmodium berghei ANKA in mice. No toxic effect or mortality was observed in mice treated, orally, with any of the extracts as a single dose, of 500 mg/kg body weight, or as the same dose given twice weekly for 4 weeks (to give a total dose of 4 g/kg). No significant lesions were observed, by eye or during histopathological examinations, in the hearts, lungs, spleens, kidneys, livers, large intestines or brains of any mouse. At doses of 200 mg/kg, all the ethanolic and dichloromethane extracts produced significant chemosuppressions of parasitaemia (of > 60% for C.occidentalis root bark and Phyllanthus niruri whole plant, and of 30% for M.morindoides leaves) when administered orally. The most active ethanolic extract, that of Phyllanthus niruri, reduced parasitaemia by 73%. The dichloromethane extracts of M.morindoides and Phyllanthus niruri produced similar reductions (74% and 72% chemosuppression, respectively), whereas that of C.occidentalis was slightly less active (60% chemosuppression). Each lyophilized aqueous extract was less active than the corresponding ethanolic extract.12

 

VI.Activity Against Filarial Mosquito (Culex quinquefasciatus) –

1.      18 plants were evaluated for juvenile hormone analogue activity against Culex   quinquefasciatus. Of these acetone extracts of 8 plants namely Commelina benghalensis , Ageratum conyzoides , Achyranthus aspera, Sida acuta, Euphorbia pulcherrina, Rivinia humilis, Ruellia tuberosa and Phyllanthus niruri possessed significant juvenile hormone activity. The LC50 values of 5 most active plants namely Phyllanthus niruri, Amaranthus spinosus, Antegonon leptopus, Corchorus aestuans, Corchorus benghalensis were determined to be 13,16,17,17,14ppm respectively.13

 

VII.Anti- spasmodic activity –

1.      Research done in Brazil at the Federal University of Santa Catarina in 1984 on   Phyllanthus niruri revealed an alkaloid (phyllanthoside) in the leaves and stem with strong antispasmodic activity.  It served as a relaxing agent for smooth muscles and they concluded that its spasmolytic action probably accounted for the efficacy of Phyllanthus niruri in expelling stones.14

VIII. Analgesic activity –

1.      Methanol extract of dried callus tissue at a concentration of 10mg/kg, administered  intraperitonially to mice was active vs. acetic acid induced writhin and vs. formalin – induced pedal edema. The extract, at 50mg/kg was inactive vs tail flick response to radiant heat. Ethanol/ water (1:1) extract of dried entire plant at a dose of 50mg/kg, administered intragastric to male mice was active. The extract also administered intraperitonially to male mice at a dose of 0.3mg/kg was active. In both cases antinociceptive effects were demonstrated using 5 different models of nociception. 15

IX. Chromosome Aberration Inhibition –

1.      Water extract of dried fruit and leaves, at a dose of 685.0 mg/kg, administered to mice   by gastric incubation was active vs. chromosome damage induced by lead nitrate and aluminium sulphate in bone marrow chromosomes. Dosing was for 7 days. 16

TOXICITY OF SAMPA-SAMPALUKAN

Abstract

Phyllanthus niruri is a plant with medicinal properties. It is often used to treat mild malaria and the elimination of renal stones. However, studies on its toxicity are scarce. The study was carried out to determine if the aqueous leaf extract of P. niruri administered to female Sprague-Dawley rats would illicit evidence of toxicity. Fifteen female rats weighing 150–200 g were divided into 3 groups. Rats in Group 1 were given a single low dose (LD) of 2000 mg/kg b.w. of the extract by oral gavage within 24 hrs. Rats in Group 2 were given a single high dose (HD) of 5000 mg/kg b.w. of the extract by oral gavage within 24 hrs. Rats in Group 3 were not given any extract but drinking water and served as the control group (C). All the rats were observed for signs of toxidromes for 14 days. On the 15th day, all the rats were sacrificed. Body organs were harvested for macroscopic examination. Urine and blood samples were drawn and analyzed. Hematological tests performed included full blood count and hemoglobin. Biochemical examinations included bilirubin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein, albumin, globulin, alkaline phosphatse (ALP), γ-glutamyltranspeptidase (GGT), urea, and creatinine. The results of the three groups were not significantly different. Examination of the various body organs did not show any abnormality. Thus no toxicity was observed at the levels administered. The LD50 of the aqueous extract is>5000 mg/kg. b.w.

Introduction

The Phyllanthus genus contains over 600 species distributed throughout the tropical and subtropical regions of the world. In the 1990s, a major reorganization of the Phyllanthus genus was conducted which classified P. amarusas a type of P. niruri (Taylor, 2003). P. niruri extract was demonstrated to block the formation of calcium oxalate crystals (Campos and Schor, 1999; Freitas et al., 2002) and stone formation in urolithiasis (Barros et al.,2003; Barros et al., 2006).
Recently, antispasmodic activity of P. niruri (Iizuka et al., 2006) and hypoglycemic effects were reported (Raphael et al., 2002; Ali et al., 2006). The hypotensive effects of P. niruri have been attributed to geraniin (Srividya & Periwal, 1995), and confirmed by its cholesterol and triglyceride lowering effects (Adeneye et al.,2006). Geraniin also possesses antiulcer properties and is believed to be seven times more potent than aspirin or acetaminophen (Miguel et al., 1996; Santos et al., 1994). The anti-malarial activity of P. niruri in 20 crude extracts from nine African medicinal plants used in Kinshasa, Congo, was confirmed (Tona et al., 1999; Cimangaet al., 2004; Mustofa et al., 2007). P. niruri and amarus are said to offer protection against HBV (Mehrotra et al., 1990), chemical toxins (Lee et al., 2006; Chatterjee et al., 2006; Wang, 2000), liver cancer (Rajeshkumar and Kuttan, 2000) and tumorigenesis (Rajeshkumar et al., 2002; Sripanidkulchai et al., 2002), although the latter is still controversial (Milne et al., 1994; Doshi et al., 1994; Thamlikitkul et al., 1991).
In this study, acute toxicity of P. niruri aqueous leaf extract was investigated because of limited information available on its toxicity, despite the widespread use of this medicinal plant.

Methods

The protocol was reviewed and approved by the Institutional Animal Care and Use Committee of the Noguchi Memorial Institute for Medical Research (NMIMR) according to the Guidelines for Animal Experimentation.

Plant material

P. niruri leaves were collected from the Kpando area in the Volta region of Ghana in the month of September. The plant was identified in its vernacular names by the farmers and confirmed to be the same as those previously authenticated by the herbarium at the University of Ghana Botany Department. A specimen was lodged at the herbarium with voucher number GC1009.

Extract preparation

The leaves were air dried at room temperature to a constant weight and ground to powder. The powder (1800 g) was boiled in 3000 ml of water for 15 minutes under atmospheric pressure and the solution was later filtered. The filtrate was lyophilized using a freeze-drying system, which yielded 33.27 g of freeze-dried material. The freeze-dried sample was stored in a cool dry place until ready for use.

Animals and experimental design

Fifteen (15) female Sprague-Dawley (S-D) rats (weighing 150–200 g) were obtained from the NMIMR. During the acclimatization period, clinical observations as well as body weight measurements of the animals were conducted and they were found healthy. The rats were assigned into groups including a control group by the stratified random method according to their body weight. S-D rats were fed standard chow diet (AIN-93G formulation, obtained from GAFCO – Ghana) ad libitum.

Housing conditions

S-D rats were housed in metal cages with stainless steel tops in the animal care facility of NMIMR, where room temperature, humidity and ventilation were controlled according to international standards. The rats were maintained in a 12-h light-cycle and were studied for 14 days. Prior to sacrifice, they were anesthetized with diethyl ether and later euthanized. All visible organs and tissues were macroscopically examined and harvested. Blood collection was by cardiac puncture.

Route of administration

The route of administration was by oral gavage in accordance with the main route of intake of P. niruri decoction by humans for medicinal purposes.

Acute toxicity test

Five S-D rats constituted a group. Thus three groups including the control group (C) were established. A single oral low dose (LD) of 2000 mg/kg b.w. and a single oral high dose (HD) of 5000 mg/kg b.w. P. niruri were reconstituted as aqueous homogenous suspensions. The administration volume was set at 900 µl/kg b.w. Group 1, the control group (C), fed normal chow diet, was gavaged 162 µl drinking water (once). Group 2, low dose group (LD) and group 3, high dose group (HD) were gavaged with the extract at a single administration with the doses indicated previously.

Clinical observations

The observation period was 14 days post administration. Clinical sings of toxidromes (rising fur, draping, tremors, excitability, miosis, mydriasis, twitching, salivation, morbidity, etc.) and mortality were observed while dosing. Thereafter, daily observations were made until the 14th day. Body weights were measured before dosing on the day of administration and weekly thereafter.

Urinalysis

Urinalysis was performed on the 15th day. Urine was collected in the morning and examined for pH, protein, glucose, ketone bodies, bilirubin, occult blood and urobilinogen.

Hematological indices

Hematological examinations were conducted on the 15th day at necropsy. Blood samples were collected into EDTA-2K tubes for immediate analysis using the SYSMEX hematology autoanalyzer (Kobe, Japan). Reagents for the hematology autoanalyzer were obtained from STROMATOLYZER (WH, USA). Leukocyte count, erythrocyte count, hemoglobin concentration, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), reticulocyte ratio, platelet count and differential leukocyte counts were determined.

Biochemical analyses

Biochemical examinations were performed using blood collected into plain tubes. Blood samples were centrifuged at 3000 rpm for 5 minutes. The serum was collected for assays. The following biochemical assays were performed using the SELECTRA JUNIOR Version 04 autoanalyzer (Vital Scientific, Spankeren, The Netherlands): total bilirubin (TBIL), direct bilirubin (DBIL), aspartate amino transferase (AST), alanine amino transferase (ALT), total protein (TP), albumin (ALB), globulin (GB), alkaline phosphatase (ALP), γ-glutamyltranspetidase (γ-GT), urea (URE), creatinine (CR).

Statistical analysis

The statistical analysis of the data was done using SPSS (Statistical Package for Social Sciences) version 17.0. All data was expressed as Mean±SD. Statistical difference was established using the independent Student's t-test for paired and unpaired data. A probability value of p ≤0.05 was considered statistically significant. For multiple groups, analysis of variance (ANOVA) was used to determine statistical differences. p-values ≤0.05 were considered significant. Multiple regression analysis was performed to determine predictive indicators of toxicity and their relationship with dependent variables.

Results

Urine analysis for pH, protein, glucose, ketone bodies, bilirubin, occult blood and urobilinogen were negative.
Hematological parameters did not show significant differences between C and LD groups. Slightly greater differences were noted for WBC of the C group (8.78×103/µl) and LD group (6.44×103/µl). Similarly, the platelet count was slightly lower for the LD group (793.6×103/µl) compared to the C group (812.8×103/µl). These differences, however, were not statistically significant. Additionally, at 5000 mg/kg b.w., slight WBC differences were observed for the C group (8.78×103/µl) and HD group (5.94×103/µl) that were insignificant.

Table 1

Table of hematological indices of the Control group, Low Dose group (LD=2000 mg/kg b.w.) and High Dose group (HD=5000 mg/kg b.w.) on day 15 after the administration of P. niruri aqueous leaf extract on Sprague-Dawley rats on day 1.

Variable
Control
LD
HD
p-value

WBC×103/ml

8.8±2.0

6.4±2.5

5.9±2.6

NS

RBC×106/ml

        6.2±0.4

6.3±1.7

         6.1±1.6

NS

HGB g/dl

        12.1±0.6

12.2±3.2

      11.9±3.2

NS

HCT%

       39.4±1.7

39.3±10.6

      38.3±10.3

NS

MCV fl

      63.3±2.0

62.9±17.0

      63.1±16.7

NS

MCH pg

      19.4±0.6

19.5±5.3

19.6±5.2

NS

MCHC g/dl

      30.7±0.4

31.0±8.5

       31.1±8.3

NS

PLT×103/ml
       813±261

794±259

     782.8±248.6

NS

LYM%
       86.5±3.1

87.3±23.4

       87.5±23.1

NS

LYM×103/ml
         7.6±1.7
        5.6±2.1
         5.2±2.3

NS

RDW-SD fl

31.8±0.6

      31.5±8.7

30.9±8.4

NS

RDW-CV%

12.6±0.3

12.3±3.4

       12.0±3.3

NS

PDW fl
       7.3±0.3

  6.9±1.9

         7.4±2.0

NS

MPV fl
       6.4±0.1
       6.1±1.7
         6.4±1.7

NS

P-LCR%
       4.4±0.6
       3.6±1.1
         4.9±1.6

NS


NS=Not Significant; WBC=White Blood Cells; RBC=Red Blood Cells; HGB=Hemoglobin; HCT=Hematocrit; MCV=Mean Corpuscular Volume; MCH=Mean Corpuscular Hemoglobin; MCHC=Mean Corpuscular Hemoglobin Concentration; PLT=Platelet; LYM%=Lymphocytes Percentage; LYM=Lymphocyte Count; RDW-SD=Standard Deviation in Red Cell Distribution Width; RDW-CV=Coefficient of Variation in Red Cell Distribution Width; PDW= Platelet Distribution Width; MPV= Mean Platelet Volume; P-LCR= Platelet Larger Cell Ratio



Table 2 shows renal function determined by urea and creatinine levels. Urea values were 7.6±1.1 mmol/l (C group), 8.1±2.1 mmol/l (LD group) and 8.7±2.3 mmol/l (HD group). The differences between the groups were insignificant. Creatinine was reduced from 67.9±9.7µmol/l (C) to 59.4±17.2 µmol/l (LD) and to 56.5±17.3 µmol/l (HD). Creatinine differences were not significant. For the liver function test, total protein, albumin and globulin showed slight yet insignificant decreases in the LD and HD groups. Total bilirubin decreased from 2.1±0.7 µmol/l (C group) to 0.5±0.1 µmol/l (LD group). However, there was a slight increase to 2.2±1.4 µmol/l when the HD extract was administered. Nevertheless, changes were not significant. Similarly did the direct and indirect bilirubin levels decline non-significantly in the LD group. In the HD group, direct and indirect bilirubin levels were virtually unchanged. ALT levels were slightly reduced in the LD group. There was a further reduction after the HD administration. However, differences were not significant. AST declined in the LD group (144±44 U/l) compared to the C group (159±42 U/l) but remained unchanged in the HD group (159±46 U/l). Changes were not significant. Mean ALP level was 444±36 U/l in the C group, 388±136 U/l in the LD group and 420±127 U/l in the HD group. The differences were insignificant. Although γ-GT increased from 1.4±0.68 U/l (C group) to 1.9±0.71 U/l (LD group), the difference was not significant. Contrary to the increase in γ-GT observed after the LD administration, γ-GT decreased from 1.4±0.68 U/l (C group) to 1.1±0.68 U/l in the HD group. The differences were not significant.

Table 2
Table of biochemical indices of the Control group, Low Dose group (LD=2000 mg/kg b.w.) and High Dose group (HD=5000 mg/kg b.w.) on day 15 after administration of P. niruri aqueous leaf extract on Sprague-Dawley rats on day 1.

Variable
Control
LD
HD
p-value
URE
mmol/l

7.6±1.1

8.1±2.1

8.7±2.3

     NS
CR
µmol/l

67.9±9.7

    59.4±17.2
    56.5±17.3
     NS
TP
g/l

60.1±5.3

  57.4±15.5

55.0±15.6

NS

ALB
g/l

36.6±2.8

35.0±9.4

    33.8±9.5
      NS
GB
g/l

23.5±2.6

 22.4±6.1

21.2±6.1

NS

DBIL
µmol/l

1.2±0.6

      0.7±0.5

1.1±0.6

     NS
IBIL
µmol/l

0.9±0.4

0.3±0.1

     1.1±1.3

 NS

TBIL
µmol/l
      2.1±0.7

0.5±0.1

    2.2±1.4
      NS
GGT
U/l
       1.4±0.7
      1.9±0.7
     1.1±0.7
      NS
ALT
U/l

129±20

     114±34

107±36

      NS
AST
U/l

159±42

144±44

159±46

NS

ALPU/l

444±36

 388±136

420±127

NS


NS=Not Significant; URE=urea; CR= creatinine; TP=total protein; ALB=albumin; GB=globulin; DBIL=direct bilirubin; IBIL=indirect bilirubin; TBIL=total bilirubin; GGT=γ-glutamyltranspeptidase; ALT=alanine aminotransferase; AST=aspartate aminotransferase; ALP=alkaline phosphatase

Discussion

International opinion and regulations relating to human health necessitate that every new pharmaceutical drug be tested for its safety before it is administered to human volunteers and patients. Toxicity studies in appropriate animal models are therefore commonly used to assess the potential health risk to humans. Such toxicity studies assess the hazard, namely the basic toxicity of the substance, and the risk is determined by considering the probability of exposure to a particular hazard at certain levels (Klaassen & Eaton, 1991). This is a key stage in ensuring the safety of drugs and an acute toxicity study is just one of the batteries of toxicity tests that are used for such purposes.
Acute toxicity tests provide preliminary information on the toxic nature of a material for which no other toxicological information is available. Such information can be used to: (i) deal with cases of accidental ingestion of a large amount of the material; (ii) determine possible target organs that should be scrutinized and/or special tests that should be conducted in repeated-dose toxicity tests; and (iii) select doses for short-term and sub-chronic toxicity tests when no other toxicology information is available (Gad & Chengelis, 1988). Furthermore, the majority of pharmaceutical companies use only acute toxicity studies to determine the minimum lethal or maximum non-lethal dose. In exceptional circumstances, the information from acute toxicity studies is used in dose-setting for other studies (NC3RS, 2007) and in such cases, the pathological examination is usually limited to macroscopic observations so that target organs are generally identified. Additionally, acute toxicity measurements help to determine the therapeutic index, i.e. the ratio between the pharmacologically effective dose and the lethal dose in the same strain and species, as well as accurately elucidate the toxicity of the medicinal plant (Klaassen & Eaton,1991). The incorporation of all available information can help in reducing the hesitation in deciding to use herbal medicinal products (HMP).
Although HMPs are widely considered to be of lower risk compared with synthetic drugs, they are not completely excluded from the possibility of having toxic or other adverse effects (De Smet, 2004). There are, however, challenges unique to HMPs. Often, deficiencies such as under-reporting of adverse reactions, general lack of toxicological information on herbs, and the quality of the reported information present challenges when signals of safety concern arise.
The lack of adequate scientific evidence on the safety of P. niruri is often a major issue to the acceptance and use of this medicinal plant. In this study, the plant was successfully identified as P. niruri and therefore the results are not extrapolated beyond this species. The absence of toxidromes was evident at the time of extract administration and thereafter. The biochemical data, mainly the hepatobiliary and renal systems, did not suggest any toxicity. Furthermore, there were no statistical differences between the low dose (2000 mg/kg b.wt.) and the high dose (5000 mg/kg b.wt.) extract administration. Hematologically, the present data did not show any adverse effect either at the low or the high dose. Thus the aqueous leaf extract of P. niruri can be considered non-toxic at the acute level and consequently, the LD50 of P. niruri aqueous leaf extract is more than 5000 mg/kg b. wt.
Because the existing literature on the toxicity of Phyllanthus niruri is limited, coupled with environmental factors including climate, soil and water changes that may have modified the chemical composition of the plant, retesting after long periods is imperative to validate any existing data in the light of newer analytical tools available. With single-ingredient products, it is important that the plant part used be identified. Knowledge of the specific plant part, associated with suspected adverse reactions or toxicity, improves assessment of previously reported adverse effects. Additionally, it must be recognized that various extraction procedures of the same herb, or plant part, produce finished products of varying chemical composition (Williamson et al., 1996) and therefore data interpretation must be judiciously assessed. From this study it is concluded that the aqueous leaf extract of P. niruri has an LD50 greater than 5000 mg/kg b.w. with no adverse effect of this dose after a single administration.

References:
1.Prajapati N.D., Purohit S.S., Sharma A.K., Kumar T.: A Handbook of Medicinal Plants- A Complete Source Book. Agrobios: India (Jodhpur); 1stEdi., 392.
2.Ross Ivan A.: Medicinal Plants of the World Chemical Constituents, Traditional & Modern Medicinal uses, Humana Press: New Jersey (Totowa); 249-252.
3.Meixa W, Haowei C, Yanjin L. et al (1995) Herbs of the genus Phyllanthus in the treatment of Chronic hepatitis B observation with three preparation from different geographic sites, J. Lab. Clin. Med.126(2): 350.
4.Thyagarajan SP, Subramanian S, Thirunalasundar T. et al (1988): Effect of Phyllanthus niruri on chronic carriers of hepatitis B virus, Lancet. 2: 764­6.
5.Latha U, Rajesh MG. (1999): Hepatoprotective effect of an Ayurvedic medicine, Indian Drugs. 36 (7): 470-473.
6.Pradhan NR. (2001): Therapeutic effect of catliv on induced hepatopalthy in calves, Indian veterinary journal. 79 (12): 1104-1106.
7.Shimizu M.(1989): Studies on aldose reductase inhibitors from natural products. II. Active components of a Paraguayan crude drug Para-parai mi, Phyllanthus niruriChem. Pharm. Bull. 37 (9): 2531-2532.
8.Naik AD, Juvekar AR. (2003): Effects of alkaloidal extract of Phyllanthus niruri on HIV replication, Indian J. Med. Sci.  57: 387-93.
9.Natarraj CG. (2000): Role of herbal extracts in HIV infected patients,Proceedings of International Congress on Ayurveda. 207.
10.Chandra R. (2000): Lipid lowering activity of  P. niruriJournal of Medicinal & Aromatic Plant Sciences. 22 (1): 29-30.
11.Raphael KR, Sabu MC, Kuttan R. (2000): Antidiabetic activity ofPhyllanthus niruriAmala research bulletin. 20: 19-25.
12.Neraliya S, Gaur R. (2004): Juvenoid activity in plant extracts against filarial mosquito Culex quinquefasciatus, Journal of Medicinal and Aromatic Plant Sciences. 26 (1): 34-38.
13.Calixto JB. (1984): Antispasmodic effects of an alkaloid extracted from Phyllanthus sellowianus: a comparative study with papaverine,Braz J.med. Biol. Res. 313-321.
14.Grewal RC.: Medicinal Plants. Campus Book International: New Delhi, 1st Edi. ,   298-304.
15.Santos AR. (1994): Analgesic effects of callus culture extracts from selected species of Phyllanthus in mice, J.Pharm. Pharmacol. 46(9): 755-759.
16.Holdsworth D. Wamoi B. (1982): Medicinal plants of the Admiralty Islands, Papau New Guinea. Part 1, Int. J.Crude Drug Res. 20(4): 169-181.
17. Halberstein RA, Saunders AB. (1978): Traditional Medicinal Practices and Medicinal plant usage on a Bahamian Island, Cul. Mad. Psychiat. 2: 177-203.
18.Ricardo MS. (1944): investigation of quinine in Phyllanthus niruri,ANALES Univ.Santo Domingo. 8: 295.
19.Singh YN. (1986): Traditional medicine in Fiji. Some herbal folk cures used by Fiji Indians, J. Ethnopharmacol. 15(1): 57 -88.
20.  Duke JA. (1975): Ethnobotanical observations on Cuna Indians, Econ. Bot. 29: 278.
21.  Weninger B, Rouzier R M, Henrys DD, Henrys JH, Anthon R. (1986): Popular medicine of Plateau of Haiti. 2 Ethnopharmacological inventory,J. Ethnopharmacol. 17(1): 13-30.
22.  Weninger B, Haag-Berrurier M, Anthon R.(1982): Plants of Haiti used as antifertility agents, J. Ethnopharmacol. 6(1): 67-84.
23.Sahu TR. (1984): Less known uses of weeds as medicinal plants,Ancient Sci. Life. 3(4): 245-249
24.Chauhan JS, Sultan M, Srivastava SK. (1977): Two new Glycoflavones from the roots of Phyllanthus niruri, Planta Med., 32, 217-222.
25.  Jain SR, Sharma SN. (1967): Hypoglycaemic Drugs of Indian Indigenous Origin, Planta Med. 15(4): 439-442.
26.  Sircar NN. (1984): Pharmacotherapeutics of Dasemani Drugs,Ancient Sci. Life. 3(3): 132-135.
27.  Holdsworth D, Gideon O, Pilokos B. (1989): Traditional medicine of New Ireland, Papua New Guinea part III, Int. J. Crude Drug Res.  27(1): 55-61.
28.  Holdsworth D, Balun L. (1992): Medicinal plants of the East and West Sepik Provinces, Papua New Guinea, Int. J. Pharmaco. 30(3): 218-222.
29.  Velazco EA. (1980): Herbal and traditional practices related to material and child health care, Rural Reconstruction Review. 35-39.
30.Loustalot AJ, Pagan C. (1949): Local “Fever” Plants tested for the presence of Alkaloids, EL Crisol.3(5): 3.
31.Khan MR, Ndaalio G, Nkunya MHH, Wevers H. (1978): Studies on the rationale of African traditional medicine Part II. Preliminary screening of medicinal plants for antigonococci activity Pak., J. Sci. Ind. Res. 27(516): 189-192.
32.Mokkhasmit MK, Swasdimongkol W, Ngarmwathana , Permphipat U.(1971): Study of toxicity of Thai medicinal plants, J. Med. Assoc. Thailand. 54(7): 490-504.
33.Kitisin T. (1952): Pharmacological studies III Phyllanthus niruri, Siriraj Hospital Gaz. 4: 641-649.
34.  Wasuwat S A. (1967): List of Thai Medicinal plants. Asrct Bangkok, Report No. 1 on Res Project. 17, A.S.R.C.T. Thailand .17: 22.
35.Oakes AJ,  Morris MP. (1958): The West Indian Weedwoman of the United States Virgin Islands, Bull Hist. Med. 32:164.
36.Asprey G F, Thornton P. (1955): Medicinal Plants of Jamaica.III, West Indian Med. J 4: 69-82.
37.Campos Alexander H, Schor Nester (1999): Phyllanthus niruri inhibits calcium oxalate endocytosis by renal tubular cells: its role in urolithiasis, Nephron. 81:393-397.
38.Matsuura Subeki H, Takahashi K, Kobayashi S, Trimurningsih C, Yoshihara T. (2005): Anti-babesial and anti-plasmodial compounds fromPhyllanthus niruriJournal of Natural Products. 68 (4): 537-539.
39.Khatoon S, Rai V, Rawat A K, Mehrotra S. (2005): Comparitive pharmacognostic studies of 3 Phyllanthus species, J. Ethnopharmacol.Oct 14.
40.  O'Neil J. Maryadle, Annsmith, Heckelman E Patricia, Obenchain R. John Jr., Gallipeau R. Jo Ann, Dárecca Ann Mary: The Merck Index. Merck Research Laboratories: New Jersey, 139, 312, 483, 599, 624, 631, 737, 1428, 1456, 5515, 9142.
41.  Chawla J, Sane R. (1997): Thin layer chromatographic differentiation of Phyllanthus species and its implication in rational search for antihepatotoxic agents from them, Indian Drugs. 34(1): 37.
42. Adeneye AA, Amole OO, Adeneye AK(2006). Hypoglycemic and hypocholesterolemic activities of the aqueous leaf and seed extract of Phyllanthus amarus in mice. Fitoterapia. 77:511–514. 
43. Ali H, Houghton PJ, Soumyanath A. (2006).Alpha-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. J Ethnopharmacol. 107:449–455.[
44. Barros ME, Lima R, Mercuri LP, Matos JR, Schor N, Boim MA. (2006) Effect of Extract of Phyllanthus niruri on crystal deposition in experimental urolithiasis. Urol Res. 34:351–357. 
45. Barros ME, Schor N, Boim MA(2003);. Effects of an aqueous extract from Phyllantus niruri on calcium oxalate crystallization in vitro. Urol Res.  30:374–379. 
46. Chatterjee SPC. (2006) Hepatoprotective effect of aqueous extract of Phyllanthus niruri on nimesulide-induced oxidative stress in vivo. Indian J Biochem Biophys. 43:299–305. 
47. Cimanga RK, Tona L, Luyindula N, Mesia K, Lusakibanza M, Musuamba CT, Apers S, De Bruyne T, Van Miert S, Hermans N, Totté J, Pieters L, Vlietinck AJ. (2004); In vitro antiplasmodial activity of callus culture extracts and fractions from fresh apical stems of Phyllanthus niruri L. (Euphorbiaceae). part 2. J Ethnopharmacol. 95:399–404. 
48. De Smet PAGM. (2004); Health risks of herbal remedies: an update. Clin Pharmacol Ther.  76:1–17. 
49. Doshi JC, Vaidya AB, Antarkar DS, Deolalikar R, Antani DH.(1994); A two-stage clinical trial of Phyllanthus amarus in hepatitis B carriers: failure to eradicate the surface antigen. Indian J Gastroenterol.  13:7–8.
50. Freitas AM, Schor N, Boim MA. The effect of Phyllanthus niruri on urinary inhibitors of calcium oxalate crystallization and other factors associated with renal stone formation. BJU Int. 2002;89:829–834.
51. Gad SC, Chengelis CP. Caldwell, N.J.: The Telford Press; 1988. Acute toxicity testing perspectives and horizons; pp. 2–4. 318, 156, 165–167, 159.
52. Iizuka T, Moriyama H, Nagai M. (2006);Vasorelaxant effects of methyl brevifolincarboxylate from the leaves ofPhyllanthus niruri . Biol Pharm Bull. ;29:177–179. 
53. Klaassen CD, Eaton DL. (1991); Principles of toxicology. In: Amdur MO, Doull JD, Klaassen CD, editors. Casarett and Doull's Toxicology: The Basic Science of Poison. New York: Pergamon Press. pp. 32–33.
54. Lee KW, Lee HJ, Lee CY. (2006);Hepatoprotective effect of Phyllanthus in Taiwan on acute liver damage induced by carbon tetrachloride. Am J Chin Med. 34:471–482. 
55. Mehrotra R, Rawat S, Kulshreshtha DK, Patnaik GK, Dhawan BN. (1990); In vitro studies on the effect of certain natural products against hepatitis B virus. Indian J Med Res. 92:133–138.
56 Miguel OG, Calixto JB, Santos AR, Messana I, Ferrari F, Cechinel Filho V, Pizzolatti MG, Yunes RA. (1996); Chemical and preliminary analgesic evaluation of geraniin and furosin isolated from Phyllanthus sellowianus . Planta Med. ;62:146–149. 
57. Milne A, Hopkirk N, Lucas CR, Waldon J, Foo Y. (1994); Failure of New Zealand hepatitis B carriers to respond toPhyllanthus amarus . N Z Med J. ;107:243. 
58. Mustofa , Sholikhah EN, Wahyuono S. (2007);In vitro and in vivo antiplasmodial activity and cytotoxicity of extracts ofPhyllanthus niruri L. herbs traditionally used to treat malaria in Indonesia. Southeast Asian J Trop Med Public Health. 38:609–615. 
59. Williamson EM, Okpako DT, Evans FJ. (1996); Pharmacological methods in phytotherapy research. Vol 1. Chichester, England: John Wiley & Sons Ltd.; Selection, preparation and pharmacological evaluation of plant material; pp. 131–154.
60. Thomas, Melody Elaine, CHANCA PIEDRA http://www.herballegacy.com)
Chanca Piedra / Phyllanthus niruri / Tropical Plant Database
61.  Phyllanthus amarus Shumacher et Thonning / Chinese names / Catalogue of Life, China
62.  Phyto-Pharmacology of Phyllanthus amarus, an overview / Pharmacologyonline 3: 202-209 (2008)

Compiled By:
Anne Camille G. Magpantay BS Pharmacy 3
Pharmacy Informatics

62 komento:

  1. 33/40 = 91.3%
    -5 too many unnecessary information
    -2 don't include all the part of a study

    good! =)

    TumugonBurahin
    Mga Tugon
    1. I want to say a big thank you to Dr Akuna for giving me unspeakable joy, 13years ago i had Hepatitis B disease and it was a headache to me as my eyes turn yellow and after giving several tries in church and mosques and traditional homes and nothing worked out, i decide to surf online for possible cure for my ailment and to my great surprise i saw lots of people speaking of how Dr Akuna is using his herbal medicine to cure their diseases namely, HIV,CANCER,DIABETES,SARS,HEART DISEASE,BRONCHITIS AND MORE that cannot be wrote mentioned, and i also visited his website and i found out truly Dr Akuna has been helping a lot of people online, and i sent email to him and he replied back to me and i told me what and what is needed to get my herbal medicine and i gave him and without any delays i received my herbal medicine after using for 2weeks i went to test myself again and there was no trace of Hepatitis B disease in my blood again. to reach Dr Akuna Email:drakunasolutiontemple@gmail.com or Visit his web page : drakunasolutiontemple.com or Call him:+2348154625070 I'm sure he will be of help to your situation.

      Burahin
    2. I want to write a little testimony about the good work of doctor ehiaguna who cured me from Hsv 1 and 2 for just 2 week with his herbal medicine, I never believe I can be normal again and have a good life like others I always regretted the day I got diagnose with the virus, I was lost of hope when my doctor told me there is no cure for it but I keep thinking and thinking about my future, if I can have kids of my own well I am so grateful for my helper who get me cured with his herbal medicine, I go online in search of anything that can help me because I can’t deal with it forever so I found this doc ehiaguna email on a blog of someone who was cured by him I quickly contact him for help and explain all my pain to him, he told me not to worry about it there is cure for real, I never believe until he send me the herbal medicine when I order for it and I have it within 4 days that is how I took the medicine for 2 week and the following week I go for test just to confirm I was 100% cured from this sickness what a miracle in my life I am so happy right now, you can also get in contact with him if you have such sickness through drehiaguna@gmail.com and also WhatsApp him +2348073908953... And He also have herbs medicine to cured the following diseases; eczema,urethra wart,chronic problems.Herpes, Cancer, Als,Hepatitis, Diabetes, HPV,Infections,ulcer  ETC

      Burahin
    3. I’m indeed very happy for the great help that Dr ehiaguna rendered to me,I was diagnosed with HSV 2 on July 7, 2018 and I have tried all possible means to get cure but all to no avail, until i saw a post in a health forum concerning herbal doctor Dr ehiaguna, who prepares herbal medicine to cure all kind of diseases/Infection including Herpes Simplex virus Type 2, at first i doubted if it was real but decided to give it a try, when i contact this herbal doctor via his email: drehiaguna@gmail.com He assured me of a cure, he prepared a herbal medicine and sent it to me via courier service, when i received this herbal medicine, he gave me instructions on how to use it, when i applied it as instructed, i was cured of this deadly virus within 14 days of usage,he told me to go for a medical check up and i went for test, I couldn't believe i was negative and the HERPES VIRUS in my body got cured, I and my family are so happy and grateful to God for using Dr ehiaguna to Liberate me. Reach Out to this great herbal doctor via his email : drehiaguna@gmail.com you can also reach him on WhatsApp +2348073908953 God bless you

      Burahin
  2. Could anyone tell me where to find this plant?

    TumugonBurahin
    Mga Tugon
    1. Hi, you can have this herbal in capsule and in tea at www.monaherbal.com

      Burahin
    2. In the backyard...I have this plants growing in our barkyard where I always remove before.

      Burahin
  3. i am using this and its perfect for diabetics like me is amiracleherbs

    TumugonBurahin
  4. Mga Tugon
    1. Hi, you can have this herbal in capsule and in tea at www.monaherbal.com

      Burahin
  5. Order a professional Sparkling White Smiles Custom Teeth Whitening System online and get BIG DISCOUNTS!
    * Up to 10 shades whiter in days!
    * Professional Results Are Guaranteed.
    * As good as your dentist.
    * Same Teeth Whitening Gel as dentists use.

    TumugonBurahin

  6. am so glad to share my testimony how DR OSUN help me conceive after several years of marriage i found it difficult to conceive and i thought is was over i will never conceive again after 6years of marriage my husband plane for divorce but with the glory of God i found a man who is bless my God with root and herbs who help me get pregnant i want to say a big thanks to him and to the world for the great help he rendered to me for making me a complete woman today now i am 5months pregnant hoping to have My own baby soon. Glory be to God.email DR OSUN and he will help you conceive via:drosunlovespelltemple@gmail.com you can call him +2348132537313

    TumugonBurahin
  7. As a sign of gratitude for how my husband was saved from hepatitis b, i decided to reach out to those still suffering from this.
    My husband was diagnosed of hepatitis b in 2013 and it was really tough and heartbreaking for me because he was my all and the symptoms were terrible, he always have Joint pain , and he always complain of Weakness of the body . we tried various therapies prescribed by our neurologist but none could cure him. I searched for a cure and i saw a testimony by someone who was cured and so many other with similar body problem, and he left the contact of the doctor who had the cure to hepatitis b. I never imagined hepatitis b has a natura cure not until i contacted him and he assured me my husband will be fine. I got the herbal medication he recommended and my husband used it and in one months he was fully okay even up till this moment he is so full of life. hepatitis b has a cure and it is a herbal cure contact the doctor for more info on drwilliams098765@gmail.com on how to get the medication. Thanks for reading my story

    TumugonBurahin
  8. I was diagnosed with HepB 3 months ago and was devastated by the news. I have been ill and sore for over 10 years, with no one being able to figure out what was wrong with me. It was always written off as a virus or infection. Then 2 years ago the severe muscle cramps started. I had them from scalp to toes, and it was when they ran the full spectrum of tests to figure the cramps out, that they discovered the HepB along with severe Vitamin D deficiency, which I link to the HepB. I was so tired and nauseous and in constant pain. I am just sick and tired of feeling sick and tired!!nothing was really working to help my condition.I went off the Sebivo (with the doctor’s knowledge) and started on hepatitis B herbal formula i ordered from Health Herbal Clinic, my symptoms totally declined over a 5 weeks use of the hepatitis B virus natural herbal formula the disease is totally reversed!! Visit there website www. healthherbalclinic. net

    TumugonBurahin
    Mga Tugon
    1. Hi, you can have this herbal in capsule and in tea at www.monaherbal.com

      Burahin
  9. As a sign of gratitude for how my husband was saved from hepatitis b, i decided to reach out to those still suffering from this.
    My husband was diagnosed of hepatitis b in 2013 and it was really tough and heartbreaking for me because he was my all and the symptoms were terrible, he always have Joint pain , and he always complain of Weakness of the body . we tried various therapies prescribed by our neurologist but none could cure him. I searched for a cure and i saw a testimony by someone who was cured and so many other with similar body problem, and he left the contact of the doctor who had the cure to hepatitis b. I never imagined hepatitis b has a natural cure not until i contacted him and he assured me my husband will be fine. I got the herbal medication he recommended and my husband used it and in one months he was fully okay even up till this moment he is so full of life. hepatitis b has a cure and it is a herbal cure contact the doctor for more info on drwilliams098675@gmail.com on how to get the medication. Thanks for reading my testimony.

    TumugonBurahin
  10. A great blog! Really Helped me understanding more about plants! There are some information that are not necessary but still a good job!

    TumugonBurahin
  11. As a sign of gratitude for how my husband was saved from hepatitis b, i decided to reach out to those still suffering from this.
    My husband was diagnosed of hepatitis b in 2013 and it was really tough and heartbreaking for me because he was my all and the symptoms were terrible, he always have Joint pain , and he always complain of Weakness of the body . we tried various therapies prescribed by our neurologist but none could cure him. I searched for a cure and i saw a testimony by someone who was cured and so many other with similar body problem, and he left the contact of the doctor who had the cure to hepatitis b. I never imagined hepatitis b has a natural cure not until i contacted him and he assured me my husband will be fine. I got the herbal medication he recommended and my husband used it and in one months he was fully okay even up till this moment he is so full of life. hepatitis b has a cure and it is a herbal cure contact the doctor for more info on drwilliams098675@gmail.com on how to get the medication. Thanks for reading my testimony.

    TumugonBurahin
  12. I want to say a big thank you to Dr Akuna for giving me unspeakable joy, 13years ago i had Hepatitis B disease and it was a headache to me as my eyes turn yellow and after giving several tries in church and mosques and traditional homes and nothing worked out, i decide to surf online for possible cure for my ailment and to my great surprise i saw lots of people speaking of how Dr Akuna is using his herbal medicine to cure their diseases namely, HIV,CANCER,DIABETES,SARS,HEART DISEASE,BRONCHITIS AND MORE that cannot be wrote mentioned, and i also visited his website and i found out truly Dr Akuna has been helping a lot of people online, and i sent email to him and he replied back to me and i told me what and what is needed to get my herbal medicine and i gave him and without any delays i received my herbal medicine after using for 2weeks i went to test myself again and there was no trace of Hepatitis B disease in my blood again. to reach Dr Akuna Email:drakunasolutiontemple@gmail.com or Visit his web page : drakunasolutiontemple.com or Call him:+2348154625070 I'm sure he will be of help to your situation.

    TumugonBurahin
    Mga Tugon
    1. Hi, you can have this herbal in capsule and in tea at www.monaherbal.com

      Burahin
  13. ALL THANKS TO DR WILLIAMS
    I was diagnosed of hepatitis B in 2011, I have tried all possible means to get cure but all my effort proved abortive, until a friend of mine introduced me to a herbal doctor , who prepare herbal medicine to cure different kind of diseases including hepatitis b virus (HBV), when i contacted this herbal doctor via his email, he sent me hepatitis b herbal medicine via courier service, when i received the herbal medicine he gave me step by step instructions on how to apply it, when i applied it as instructed i was totally cured from this disease within 1 months of usage. any body with similar problem can Contact this great herbal doctor via his email drwilliams098675@gmail.com for advice and for his product,and thanks to you admin for such an informative blog.

    TumugonBurahin
    Mga Tugon
    1. Hi, you can have this herbal in capsule and in tea at www.monaherbal.com

      Burahin
  14. Naalis ng may-ari ang komentong ito.

    TumugonBurahin
  15. Naalis ng may-ari ang komentong ito.

    TumugonBurahin
  16. How to use the boiling leaves and stem for breaking my gallstone.. thank you

    TumugonBurahin
    Mga Tugon
    1. Hi, you don't need to boil this plant, because we have capsule and in tea at www.monaherbal.com

      Burahin
  17. There is a great herbal man call Dr okougbo who can cure HERPES VIRUS and other deadly diseases with the use of natural herbs. He is from Africa and he is a great herbal doctor and he can also cure you as well if you are having the virus And other deadly disease and here is is email address dr.okougbospellhome@gmail.com or Whatsapp he +2349032690878
    HERPES
    HIV/AIDS
    HEPATITIS
    BAD BREATH

    TumugonBurahin
  18. We are selling this herbal for a years, in capsule and in tea. Please visit mga kabayan to buy and restore your healthy lifestyle. www.monaherbal.com

    TumugonBurahin
  19. I am here to testify how Dr banlogun herbal medicine helped my son,my son once had the problem of hapetitis B which affected his Education for years,i gave him different medication but there was no solution,i search the net and i found the contact of a doctor,who help me with his product on curing HIV/AIDS, CANCER< HERPES, HEP B, DIABITIES, HSV, MOUTH ODOUR, STD, VAGINAL DISEASE/ ITCHING,, CHRONIC HEPATITIS,GERNITAL WARTS, HPV, EPILEPSY, OVARIAN CANCER/CYST, BLOOD PRESSURE, STROKE, BAD BREATH problem and other deadly diseases,he also assured me that it is a permanent cure,my son took the medication for just 2 weeks and he was back to normal. for two years now he has been doing fine.I give all thanks to Dr banlogun for helping me out of such problem now am the hapiest person on earth for seeing my son doing better again.you can also contact him on Drbanlogunsolutionhome@gmail.com you can also call or whatsapp on +2349032508349 or +2349053287594and get the product.

    TumugonBurahin
  20. As a sign of gratitude for how my husband was saved from hepatitis b, i decided to reach out to those still suffering from this.
    My husband was diagnosed of hepatitis b in 2013 and it was really tough and heartbreaking for me because he was my all and the symptoms were terrible, he always have Joint pain , and he always complain of Weakness of the body . we tried various therapies prescribed by our neurologist but none could cure him. I searched for a cure and i saw a testimony by someone who was cured and so many other with similar body problem, and he left the contact of the doctor who had the cure to hepatitis b. I never imagined hepatitis b has a natural cure not until i contacted him and he assured me my husband will be fine. I got the herbal medication he recommended and my husband used it and in one months he was fully okay even up till this moment he is so full of life. hepatitis b has a cure and it is a herbal cure contact the doctor for more info on drwilliams098675@gmail.com on how to get the medication. Thanks for reading my testimony.

    TumugonBurahin
  21. Just wanted to say a huge thank you to Dr Williams for everything he have done in my life. I have been in tears for so many years now, having found that my husband is suffering from hepatitis b, lesion after some years of being under extreme stress., . I spent 24 hours freaking out and finally calmed down after reading some testimony on here about Dr Williams herbal medicine to hepatitis b . The moment came and I mumbled out to my husband about Dr Williams product (with a few tears) and he was AMAZE – . He was just worried about his health, been free from this illness, that was how will order for Dr Williams product on line , after my husband finish taking Dr Williams medicine for just within a period of one month. Will went to the doc together who confirmed that he was hepatitis b free.
    Thank you again for being upfront, truthful and taking this pain away from my family for more information about his product you can email him on drwilliams098675@gmail.com i hope you can also help other terrified people in a similar situation.

    TumugonBurahin
  22. where can i acquire this plant?

    P.S cause i really need it for my research

    TumugonBurahin
  23. how many leaves are to boil..for hepa b

    TumugonBurahin
  24. Am really grateful and thankful for what Dr adigba has done for me and
    my family. I Was having Hepatitis for good three years with no solution,
    the diseases almost took my life and because I was unable to work and
    I was also loosing lots of money for medication, but one faithful day
    when I went online, I met lots of testimonies about this great man so I
    decided to give it a try and to God be the glory he did it. he cured me
    of my diseases and am so happy and so pleased to Write about him
    today. if you need his help or you also want to get cured just the way I
    got mine, just email him below
    dradigba@gmail. com or Whatsapp ;+2348077875210 and
    get your healing. He has cure for other deadly diseases like Diabetes,
    Hiv/Aids, Hepatitis of all types and Cancer

    TumugonBurahin
  25. i want to inform the public how i was cured of HERPES Simplex Virus by a Doctor called Dr.EBHOTA. i visited different hospital but they gave me list of drugs like Famvir, Zovirax, and Valtrex which is very expensive to treat the symptoms and never cured me. I was browsing through the Internet searching for remedy on HERPES and i saw comment of people talking about how Doctor EBHOTA cured them. I Was scared because i never believed in the Internet but i was convince to give him a try because i was having no hope of been cured of HERPES so i decided to contact him on his email, i searched his email on net and i saw a lot of people testifying about his goodness. when i contacted him he gave me hope and send a Herbal medicine to me that i took and it seriously worked for me, am a free person now without problem, my HERPES result came out negative. You can contact him on
    his Email: drebhotasolution@gmail.com or whatsapp him on +2348089535482 and get all your problem solved.
    1Sickness of any kind
    2 Help to CURE Hepatitis B, HIV/AIDs CANCER and BIPOLAR
    3 Divorce, Breakup problem and To Re-unit
    4 Pregnancy problem
    5 Financial problem and Job promotion
    6 To get a good and rich life partner
    7 To Lose your wath
    8 to enlargement of pennis
    9. CANCER
    10. ALS (Lou Gehrig's disease)
    11 Hepatitis B
    12 chronic pancreatic
    13 emphysema
    14 COPD (chronic obstructive pulmonary disease)   

    TumugonBurahin
  26. I am here to give my testimony about Dr Ebhota who helped me.. i want to inform the public how i was cured from (HERPES SIMPLEX VIRUS) by salami, i visited different hospital but they gave me list of drugs like Famvir, Zovirax, and Valtrex which is very expensive to treat the symptoms and never cured me. I was browsing through the Internet searching for remedy on HERPES and i saw comment of people talking about how Dr Ehbota cured them. when i contacted him he gave me hope and send a Herbal medicine to me that i took for just 2 weeks and it seriously worked for me, my HERPES result came out negative. I am so happy as i am sharing this testimony. My advice to you all who thinks that there is no cure for herpes that is Not true just contact him and get cure from Dr Ebhota healing herbal cure of all kinds of sickness you may have like
    (1) CANCER,
    (2) DIABETES,
    (3) HIV&AIDS,
    (4) URINARY TRACT INFECTION,
    (5) CANCER,
    (6) IMPOTENCE,
    (7) BARENESS/INFERTILITY
    (8) DIARRHEA
    (9) ASTHMA
    (10)SIMPLEX HERPES AND GENITAL
    (11)COLD SORE
    HERPES. he also cure my friend from cervical cancer Email: drebhotasoultion@gmail.com or whatsapp him on +2348089535482

    TumugonBurahin
  27. FINALLY FREE FROM HERPES VIRUSI thought my life had nothing to offer anymore because lifebecame meaningless to me because I had Herpes virus, thesymptoms became very severe and bold and made my familyrun from and abandoned me so they won't get infected. I gaveup everything, my hope, dreams,vision and job because thedoctor told me there's no cure. I consumed so many drugs butthey never cured me but hid the symptoms inside me makingit worse. I was doing some research online someday when Icame across testimonies of some people of how DR Ebhotacured them from Herpes, I never believed at first and thoughtit was a joke but later decided to contact him on the detailsprovided and when I messaged him we talked and he sent mehis herbal medicine and told me to go for a test after twoweeks. Within 7 days of medication the symptomsdisappeared and when I went for a test Lo and behold I wasNEGATIVE by the Doctor Who tested me earlier. Thank you DREbhota because I forever owe you my life and I'll keep ontelling the world about you. If you are going through samesituation worry no more and contact DR Ebhota viadrebhotasolution@gmail. com or WhatsApp him via +2348089535482.he also special on cureing 1. HIV/AIDS2. HERPES 3. CANCER 4.ALS 5. HEPATITIS B 6.DIABETES 7. HUMAN PAPILOMA VIRUS DISEASE(HPV)8. ALZHEIMER 9. LUPUS (Lupus Vulgaris or LupusErythematosus 

    TumugonBurahin
  28. Hello viewer’s
    I don’t have much to say there are so may scammers going on online so we cant detect the real herbal medicine doctors. Thank GOD for leading me, please don’t ignore this post is real Dr Emmanuel, is a real herbal Doctor, he cured me from Herpes virus, i am living so happy and free , i was fully recovered within 4 weeks of usage of dr Emmanuel herbal medicine ,please viewers out there that have any deadly disease don't fail to contact him via his email; nativehealthclinic@gmail.com or WhatsApp/Call: +2348140073965  thanks once again to dr Emmanuel GOD bless you abundantly  
    he also cured HIV/AIDS,CANCER,GENITAL WART,COPD,BRAIN TUMOR

    TumugonBurahin
  29. I want to write a little testimony about the good work of doctor ehiaguna who cured me from Hsv 1 and 2 for just 2 week with his herbal medicine, I never believe I can be normal again and have a good life like others I always regretted the day I got diagnose with the virus, I was lost of hope when my doctor told me there is no cure for it but I keep thinking and thinking about my future, if I can have kids of my own well I am so grateful for my helper who get me cured with his herbal medicine, I go online in search of anything that can help me because I can’t deal with it forever so I found this doc ehiaguna email on a blog of someone who was cured by him I quickly contact him for help and explain all my pain to him, he told me not to worry about it there is cure for real, I never believe until he send me the herbal medicine when I order for it and I have it within 4 days that is how I took the medicine for 2 week and the following week I go for test just to confirm I was 100% cured from this sickness what a miracle in my life I am so happy right now, you can also get in contact with him if you have such sickness through drehiaguna@gmail.com and also WhatsApp him +2348073908953... And He also have herbs medicine to cured the following diseases; eczema,urethra wart,chronic problems.Herpes, Cancer, Als,Hepatitis, Diabetes, HPV,Infections,ulcer  ETC

    TumugonBurahin
  30. Hello, I want to write a little review about the good work of doctor Ojamo, who cured me from HSV 1&2 for just 2 week with his herbal medicine, I never believe I can be cured again and have a good life like others, I always regretted the day I got diagnose with this virus, I become lost of hope when my doctor told me there is no cure for it, Well I am so grateful for my helper who get me cured with his herbal medicine, I went online in search of anything that can help me because I can’t deal with it forever so I found dr Ojamo email dr.ojamoherbalhome@gmail.com on a blog of someone who was cured by him,of the same herpes, so I quickly contacted him for help and explained all my pains to him, he told me not to worry about it there is a cure for real, I never believe until he sent me the herbs when I ordered for it and I got it within 5 working days that is how I took the medicine for 2 weeks and the following week I went for a test just to confirm I was 100% cured from this sickness what a miracle in my life, I am so happy right now, and forever greatful, you can also get in contact with him if you have such sickness through his whatsapp +2349077406037 or email address:: dr.ojamoherbalhome@gmail.com

    TumugonBurahin
  31. Hello, I want to write a little review about the good work of doctor Ojamo, who cured me from HSV 1&2 for just 2 week with his herbal medicine, I never believe I can be cured again and have a good life like others, I always regretted the day I got diagnose with this virus, I become lost of hope when my doctor told me there is no cure for it, Well I am so grateful for my helper who get me cured with his herbal medicine, I went online in search of anything that can help me because I can’t deal with it forever so I found dr Ojamo email dr.ojamoherbalhome@gmail.com on a blog of someone who was cured by him,of the same herpes, so I quickly contacted him for help and explained all my pains to him, he told me not to worry about it there is a cure for real, I never believe until he sent me the herbs when I ordered for it and I got it within 5 working days that is how I took the medicine for 2 weeks and the following week I went for a test just to confirm I was 100% cured from this sickness what a miracle in my life, I am so happy right now, and forever greatful, you can also get in contact with him if you have such sickness through his whatsapp +2349077406037 or email address:: dr.ojamoherbalhome@gmail.com

    TumugonBurahin
  32. Here is  my testimony on how I was cured of HIV by Dr Akhigbe,with his natural herbal medicine.  on a regular basis in efforts to help others when I could. As you may know, each donation is tested.  Well, on July 6th I had a meeting with a Red Cross representative and was told that I had HIV. “What went through your mind when you heard that "Rose" Good question reader! To be honest, I thought my life was over, that I would ever find love, get married, have children or anything normal. Lucky for me I have an amazing support system.  My family supported me then I never thought that I was invincible to STD s or pregnancy or anything else parents warn their kids about. I just didn’t think about it. I was in a monogamous relationship and thought that I asked the right questions.  We even talked about marriage Scary.  During that time I was in college and donated blood on a re as well. who helped me in search of cure through the media.there we saw a good testimony of sister 'Kate' about the good work of Dr Akhigbe natural herbal medicine cure.then I copied his email address and contacted him. A good herbalist doctor with a good heart, he is kind, loving and caring. He replied back to my message and told me what to do. After a week the doctor sent me my herbal medicine and instructed me how to take it.Yes it worked very well, after drinking it I went to the hospital for another test and everything turned negative. What a wonderful testimony I can never forget in my life. Dr Akhigbe is a man who gave me a life to live happily forever so all I want you all to believe and know that cure of HIV is real and herbs is a powerful medicine and it works and heals.  Dr Akhigbe also used his herbal medicine to cure diseases like:   HERPES, DIABETES, SCABIES, HEPATITIS A/B, STROKE, CANCER, ALS, TUBERCULOSIS, ASTHMA, PENIS ENLARGEMENT, MALARIA, LUPUS, LIVER CIRRHOSIS, DEPRESSION, HIV/AIDS, EPILEPSY, BACTERIAL, DIARRHEA, HEART DISEASES, HIGH BLOOD PRESSURE, PARKINSON'S, ALZHEIMER, COLD URTICARIA, HUMAN PAPILLOMAVIRUS,INSOMNIA,  BACTERIAL VAGINOSIS, SCHIZOPHRENIA, JOINT PAIN, STOMACH PAIN, CHROME DISEASES, CHLAMYDIA, INSOMNIA HEARTBURN, ,  THYROID, MAR BURG DISEASES, MENINGITIS, ARTHRITIS, BODY WEAK, SMALLPOX, DENGUE, FEVER, CURBS, CHANCRE, AND OTHERS VARIOUS DISEASES/ VIRUS.   You are there and you find yourself in any of these situations, kindly contact Dr Akhigbe now to help you get rid of it. Here is his email address:
       drrealakhigbe@gmail.com      or you can write to him on whats app with his phone number:   +2349010754824.
      My appreciation is to share his testimony for the world to know the good work Dr Akhigbe has done for me and he will do the same for you.
       

    TumugonBurahin
  33. I am here to give my testimony about Dr Ebhota who helped me.. i want to inform the public how i was cured from (HERPES SIMPLEX VIRUS) by salami, i visited different hospital but they gave me list of drugs like Famvir, Zovirax, and Valtrex which is very expensive to treat the symptoms and never cured me. I was browsing through the Internet searching for remedies on HERPES and I saw comments of people talking about how Dr Ehbota cured them. When I contacted him he gave me hope and sent Herbal medicine to me that I took for just 2 weeks and it seriously worked for me, my HERPES result came out negative. I am so haas I am sharing this testimony. My advice to you all who thinks that there is no cure for herpes that is Not true just contact him and get cure from Dr Ebhota healing herbal cure of all kinds of sickness you may have like (1) CANCER,(2) DIABETES,(3) HIV&AIDS,(4) URINARY TRACT INFECTION,(5) CANCER,(6) IMPOTENCE,(7) BARENESS/INFERTILITY(8) DIARRHEA(9) ASTHMA(10)SIMPLEX HERPES AND GENITAL(11)COLD SOREHERPES. he also cured my friend from cervical cancer Email: drebhotasoultion@gmail.com or whatsapp him on +2348089535482

    TumugonBurahin
  34. I have been suffering from (HERPES SIMPLEX VIRUS) disease for the past one years and had constant pain, especially in my knees. During the first year,I had faith in God that i would be healed someday.This disease started circulating all over my body and i have been taking treatment from my doctor, few weeks ago i came on search on the internet if i could get any information concerning the prevention of this disease, on my search i saw a testimony of someone who has been healed from (HERPES SIMPLEX VIRUS) by this Man Dr momoh and she also gave the email address: dr.momoh9@gmail.com of this man and advise we should contact him for any sickness that he would be of help, so i wrote to Dr. momoh telling him about my (HERPES Virus) he told me not to worry that i was going to be cured!! hmm i never believed it,, well after all the procedures and remedy given to me by this man few weeks later i started experiencing changes all over me as the doctor assured me that i have cured,after some time i went to my doctor to confirmed if i have be finally healed behold it was TRUE, So friends my advise is if you have such sickness or any other at all you can email:: dr.momoh9@mail.com or whatsApp his number on+2347083724098 

    TumugonBurahin
  35. Dr.Vincent has really made me so much believe in him by getting me cured of Herpes virus with his herbal treatment. I really appreciate you Dr. Vincent for bringing back happiness to my life again. thank you so much, friends join me to thank Dr Vincent for what he has actually done for me i pray to you all for a good life, dr.vincentherbalhome6@gmail.com or you can what'sapp his mobile number +2349159559277) or go through his website https://drvincentherbalhom.wixsite.com/welcometodrvincenth Thanks Dr .vincent for your help in my life and family...

    TumugonBurahin
  36. what a miracle i never believe there is cure because my doctor tested me HSV 2 positive and she told me there is no cure, i’m very happy today that i’m having a free life without this sickness, i can remember some months ago when i was  crying all through the night and day that i can’t get cured from this sickness, i found this herbal man email on internet when i was  doing research on cure for HSV 2 i contacted him to found out if i can get help from this sickness, i was so surprise when he told me that he have the herbs cure to it and he sent me the herbal in less down 4 days i was so happy when i get someone giving me hope that he can cured me i took the herbal for just 2 weeks, when i went for test after taking the herbal i found out that i am cured i was so happy and surprise, i want to use this opportunity to inform you that there is cured to HSV 2 you can also contact him for his help as soon as possible so that you can get rid of this sickness once and for all you can reach him through this email: drehiaguna@gmail.com  or and you can also call or whatapp him now +2348073908953 You can also contact him on any sickness in this he all have the herbal cure to it  .HIV.HEPATITIS.ANTHRAX.HPV.CANCER.ALS  

    TumugonBurahin
  37. I want to write a little testimony about the good work of doctor ehiaguna who cured me from Hsv 1 and 2 for just 2 week with his herbal medicine, I never believe I can be normal again and have a good life like others I always regretted the day I got diagnose with the virus, I was lost of hope when my doctor told me there is no cure for it but I keep thinking and thinking about my future, if I can have kids of my own well I am so grateful for my helper who get me cured with his herbal medicine, I go online in search of anything that can help me because I can’t deal with it forever so I found this Dr ehiaguna email on a Facebook of someone who was cured by him I quickly contact him for help and explain all my pain to him, he told me not to worry about it there is cure for real, I never believe until he send me the herbal medicine when I order for it and I have it within 4 days that is how I took the medicine for 2 week and the following week I go for test just to confirm I was 100% cured from this sickness what a miracle in my life I am so happy right now, you can also get in contact with him if you have such sickness through drehiaguna@gmail.com and also WhatsApp him +2348073908953. he can also help with this following sickness DIABETES CURE/EPILESY/HPV/CURE/LUPUS/HEPATITIS

    TumugonBurahin
  38. I’m indeed very happy for the great help that Dr ehiaguna rendered to me,I was diagnosed with HSV 2 on July 7, 2018 and I have tried all possible means to get cure but all to no avail, until i saw a post in a health forum concerning herbal doctor Dr ehiaguna, who prepares herbal medicine to cure all kind of diseases/Infection including Herpes Simplex virus Type 2, at first i doubted if it was real but decided to give it a try, when i contact this herbal doctor via his email: drehiaguna@gmail.com He assured me of a cure, he prepared a herbal medicine and sent it to me via courier service, when i received this herbal medicine, he gave me instructions on how to use it, when i applied it as instructed, i was cured of this deadly virus within 14 days of usage,he told me to go for a medical check up and i went for test, I couldn't believe i was negative and the HERPES VIRUS in my body got cured, I and my family are so happy and grateful to God for using Dr ehiaguna to Liberate me. Reach Out to this great herbal doctor via his email : drehiaguna@gmail.com you can also reach him on WhatsApp +2348073908953 God bless you

    TumugonBurahin
  39. Herpes has been one of the most significant virus in the US now,and its spreading really fast,and the government are only producing medical drugs that can suppress it but rather keep on eliminating the African herbal doctors who were able to discover a way to completely cure the virus...I'm delighted to be finally cured of herpes 2 after i applied Dr Odiagbe herbal medication for just three weeks, he also have herbal remedies for chronic illnesses!!!  drodiagbeherbalhome82@gmail.  com

    TumugonBurahin
  40. I started on COPD Herbal treatment from Ultimate Life Clinic, the treatment worked incredibly for my lungs condition. I used the herbal treatment for almost 4 months, it reversed my COPD. My severe shortness of breath, dry cough, chest tightness gradually disappeared. Reach Ultimate Life Clinic via their website www.ultimatelifeclinic.com . I can breath much better and It feels comfortable!

    TumugonBurahin
  41. Goji Berries... yes... I tried those too.For my health conditions, Those sweet, red berries seemed to help, but only during the time when I consumed them. I don't want to take a drug for the rest of my life, so why would I want to take a natural supplement everyday for the rest of my life (although Goji berries are very tasty and are highly nourishing). To me this was not a cure either (and I'm LOOKING for the CURE).
    Up to that point, I hadn't found a cure. I felt like a young jumbled mess. I continued to have extreme pain, but continued on my path to healing. I started to focus on myself and not everyone else. When I was a young adult, I took on too much responsibility out of a sense of obligation. This was no longer healthy for me, so I resigned from all my projects and groups. Those days to come were the best [and worst] days. I took a lot of time off work, yet begun to feel so extremely exhausted. Many health professionals "diagnosed" me with adrenal fatigue & Hiv,Prostate Cancer so my situation was annoying then I keep searching for permanent cure online that's when I came to know of Dr Itua herbal center hands whom god has blessed with ancestral herbs and a gift to heal people with disease like . Cancers, Alzheimer's disease,HPV,Men & Women Infertility,Melanoma, Mesothelioma, Diabetes, Multiple myeloma, Parkinson's disease,Neuroendocrine tumors, Herpes, Hiv/Aids,Non-Hodgkin's lymphoma,, chronic diarrhea, COPD,Love spell, Hepatitis. .. So I made a purchase of his herbal medicines and I have been watching my health for 6 years now and I actually confirmed that his herbal medicines are a permanent cure and I'm so happy that I came to know of his herbal healings.
    You can contact Dr Itua herbal center Email: drituaherbalcenter@gmail.com WhatsApp: +2348149277967. if you went through exactly what I go through in terms of health conditions because to be honest there is more to learn about natural herbs than medical drugs.

    TumugonBurahin


  42. I saw so many testimonies about Dr Itua a great HERBAL DOCTOR that can cure all kind of diseases and give you the rightful health to live a joyful life, I didn't believe it at first, but as the pain got worse and my life was at risk after visiting my therapist numerous times for combination of treatments. And no changes so I decided to take a try, I contacted him also and told him I want a cure for Vulvar cancer/ Testicular cancer and it was Stage IIIA, he gave me advice on what I must do and he delivered it to me in my state which I use according to his instruction, and today I must say I am so grateful to this man Dr Itua for curing me from Vulvar cancer/ Testicular cancer and for restoring me back to my normal health and a sound life, I am making this known to everyone out there who have been living with cancers all his life or any sick person should not waste more time just contact him with his details below- WhatsApp- +2348149277967 Email drituaherbalcenter@gmail.com believe me this man is a good man with Godly heart, this is the real secret we all have been searching for. Do not waste more time contacting him today for you to also live a sound and happy life. He cures the following disease, thyroid Cancer, Uterine cancer, Fibroid, Arthritis, Brain Tumor,Fibromyalgia, Bladder cancer, Brain cancer, HIV, Herpes, Esophageal cancer, Gallbladder cancer, Gestational trophoblastic disease, Head and neck cancer, Hodgkin lymphoma Intestinal cancer, Kidney cancer,Hpv, Lung cancer, Melanoma,Mesothelioma, Multiple myeloma,Neuroendocrine tumors Non-Hodgkin lymphoma, Oral cancer, Ovarian cancer, Sinus cancer, Hepatitis A, B/C, Skin cancer, Soft tissue sarcoma, Stroke, Lupus, Spinal cancer, Stomach cancer, Vaginal cancer,Vulvar cancer, Testicular cancer,Tach Diseases, Pancreatic Cancer, Leukemia, Liver cancer, Throat cancer, Alzheimer's disease, Chronic Diarrhea,Copd, Parkinson,Als,Adrenocortical carcinoma Infectious mononucleosis.

    TumugonBurahin
  43. Dr. Ehimare has really made me so much believe in him by getting me cured of Herpes with his herbal medicine. I have been a Herpes virus patient for some time now, I have been running here and there looking for help/cure but none could help. I ran into a testimony by someone who testified how she was cured of a terrible disease by Dr. Ehimare, so I decided to contact the herbal doctor. I explained to him, and he said he can cure me. We started the process and he prepared herbs and sent them to me in my country and gave me directions on how I am to use them. I followed his directions and now To God be the glory he could cure me with his herbal mixture. I really appreciate you Dr. Ehimare for bringing back happiness to my life again. thanks you so much friends that has join me to thank Dr. Ehimare for what he has actually done for me i pray to you all for a good life and property, his contact. Email address drehimare3@gmail.com , via whats-app +1 (267) 691-1087 Thanks Dr. Ehimare for your help in my life and family... and most especially to you Dr. THANKS. 

    TumugonBurahin
  44. I have been suffering from a deadly disease (Hsv) for the past 2 years now, I had spent a lot of money going from one places to another, from churches to churches, hospitals have been my home every day residence. Constant checks up have been my hobby not until this faithful day, I was searching through the internet, I saw a testimony on how Dr Ehimare helped someone in curing his (Hsv) herpes disease, quickly I copied his email which is drehimare3@gmail.com just to give him a test I spoke to him, he asked me to do some certain things which I did, he told me that he is going to provide the herbal cure to me, which he did, then he asked me to go for medical checkup after some days after using the herbal cure, behold I was free from the deadly disease, he only asked me to post the testimony through the whole world, faithfully am doing it now, please brothers and sisters, he is great, I owe him in return. if you are having a similar problem just email him on Drehimare3@gmail.com Or whatsapp him via +1 (267) 691-1087

    TumugonBurahin
  45. I want to share my testimonies to the general
    public on how this great man called Dr OLOKUN who cure my herpes. I have been a HERPES patient for over 9 Months and i have been in pains until i came across this lady when i traveled to Africa for business trip who happer and she told me that there is this great herbal doctor that help her tocure her herpes and she gave me his email address for me to contact and i did as she instructed. The man Dr OLOKUN told me how much to buy the
    herpes herbal medication and how i will get it, which i did. And to my
    greatest surprise that i took the Herpes herbal medicine for just two week as he
    instructed and behold i went for a herpes test, to my greatest surprise for the
    doctor confirmed me to be herpes free and said that i no longer have
    herpes in my system and till now i have never felt any pains nor herpes again, so i
    said i must testify the goodness of this man to the general public. So if
    you are there surfing from this Herpes problems or any deadly disease or
    other disease for i will advice you to contact him on his working
    email: drolokuntemple@gmail.com OR on his cell phone +2348132537313 whatsapp number
    +2348132537313



    TumugonBurahin
  46. I am really happy that i have been cured from (HERPES SIMPLEX VIRUS) with the herbal medicine of  Dr MOMOH , i have been suffering from this disease for the past 3 years and 4 mouth without solution until i came across the email of this doctor who have cure so many people with his herbal medicine, i also choose to give him a chance to help me and my husband, he told me what to do and i kindly did it, and he gave us his herbal medicine and direct me on how to use it, i also follow his instructions for use and he ask us to go for a check up after 1 week and 5days which i did, to my greatest surprise our result came out as negative, we are really happy that there is someone like this doctor who is ready to help anytime any day. To all the readers and viewers that is doubting this testimony, stop doubting it and contact this doctor if you really have one and see if he will not actually help you. i am not a stupid woman that i will come out to the public and start saying what someone have not done for me and i know that there are some people out there who are really suffering and hurting their family just because of these diseases so you can Email him on dr.momoh9@gmail.com OR
    WHATSAPP him on +2347083724098  he also told me that he has cure for these diseases listed below .
    HERPES SIMPLEX VIRUS.
    .DIABETES .
    CANCER
    .HEPATITIS
    .HIV/AIDS
    Thank you for your time

    TumugonBurahin


  47. Am jeremiah, I am testifying about a great hebal man that cured my wife of hepatitis B and liver cirhosis. his name is Dr oniha. My wife was diagnose of hepatities two years ago, i almost spent all i had then, until i saw dr oniha recommendation online, and i call him, then he told me how to get the herb. You can call him on +2347089275769 or email him at dronihaspell@yahoo.com

    TumugonBurahin
  48. This is an amazing blog where I found healthcare tips and find weight issue solution by apple cider vinegar gummies for weight loss safe to use according to prescription.

    TumugonBurahin